I am reminded of that line in P&P, where Elizabeth laments,

"A man who has once been refused! How could I ever be foolish enough to expect a renewal of his love? Is there one among the sex, who does not protest against such a weakness as a second proposal to the same woman! There is no indignity so abhorrent to their feelings!"

It is not entirely clear to me just what it was in that era that made pursuing a woman who had once rejected you so difficult for a man. There must be something in it though, because Anne has absolutely no expectations in all the scenes at Uppercross, that he might still be interested in her. And from his behaviour, I think it was perfectly expected by both parties that he should not.